Def-HEP A1ATD cell morphology
The Def-HEP A1ATD cells are highly functional human hepatocytes. When thawed and plated as a monolayer Def-HEP A1ATD cells form with typical hepatocyte cobblestone morphology.
Figure 2. Clear cobblestone morphology of Cryo-Def HEP (A1AT) post thaw and recovery. Cell seeding density: 1×106 cells/mL in collagen type 1 coated 24 well plate. Confluent monolayer. Magnification level: x10.
Hepatocyte maturation markers
Def-HEP A1ATD cells display the functional characteristics of primary human hepatocytes (PHH) including albumin and A1AT production (Figure 2). Accordingly, they should be handled in a similar fashion to primary human hepatocytes.
Figure 3. Immunostaining for expression of A1AT polymers (2c1mAb) and total A1AT in Def-HEP A1ATD and PHH.
Detection of disease markers via ELISA
ELISA for polymer and secreted A1AT using antibodies specific for A1AT polymers (2C1mAb, top) or all conformers of A1AT (bottom).
Figure 4. Def-HEP A1ATD represents the cryopreservation sample. Primary human hepatocytes (PHH) and hepatocellular liver carcinoma cells (HepG2) are used as internal controls. PHH and HepG2 show A1AT polymer levels below the limit of quantification.
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