With the ability to reliably generate functionally mature human hepatocytes from iPSCs, DefiniGEN brings all the advantages of iPSC technology to hepatocyte studies - cell models that possess human-relevant physiology and that are available in virtually unlimited amounts with the same genetic background.
DefiniGEN's human hepatocytes remain functionally stable over a prolonged period of time in culture, making them ideal for drug discovery, drug metabolism, and toxicology-related studies. They also express key hepatitis markers such as CD81, SR-B1, Claudin-1 and Occludin at similar levels to primary human hepatocytes, making them an effective model for hepatitis lifecycle studies.
- Highly standardized with > 98% functionally mature human hepatocytes
- Reliable, consistent peformance delivers biologically relevant data
- Verified wild-type donor genetics and karyotype
- Stable function in culture over a +20 day window
Normal physiology includes:
- CYP450 induced activities
- Expression of hepatocyte proteins, including A1AT, ALB, HNF4a
- Secretion of physiologically relevant levels of albumin and urea
- Expression of a range of key hepatitis B & C markers
- Uptake of LDL
|Product ID||Def-HEP WT|
|Cell number||4-6 million cells|
|Pricing||Request a quote|
Hepatocyte cell morphology
When thawed and plated as a monolayer, Def-HEP cells form hepatocytes with characteristic cobblestone morphology and tight cell junctions
Hepatocyte maturation markers
QPCR analysis shows Def-HEP cells show key hepatocyte markers at similar levels to PHH. Functional characteristics including albumin secretion, A1AT production, glycogen storage and LDL uptake are also present.
Extended culture time
Multiple Inducible CYP450 Activities
Def-HEP cells display CYP450 induced activity profiles that are similar to PHH (CYP1A2 EROD assay, inducer - omeprazole), (CYP3A4 PGlo assay, inducer - rifampicin).
Hepatitis marker analysis
As assessed by dosing Def-HEP WT with valinomycin (0.3-600nM) and papverine (0.03 – 60µM) for 48 hrs both compounds caused significant mitochondrial toxicity with concentration ranges recognized in literature. Cell viability was assessed via the CellTiter 96® Aqueous Non-Radioactive Cell. Both valinomycin and papaverine caused significant mitochondrial toxicity