The cells display similar functional characteristics of primary human hepatocytes, and their functionality remains stable over a prolonged period of time in culture making them ideal for drug discovery, drug metabolism, and toxicology-related studies. These hiPSC-derived hepatocytes express key hepatitis markers such as CD81, SR-B1, Claudin-1 and Occludin at similar levels to PHH making them an effective model for hepatitis lifecycle studies.
- Highly standardized cell product containing >98% human hepatocyte cells with consistent performance and biologically relevant data
- Wild-type donor genetics and karyotype verified
- Cells are functional and stable over a +20 day window
- Cells display CYP450 induced activities
- Cells express key hepatocyte proteins, including A1AT, ALB, HNF4a,
- Cells secrete physiologically relevant levels of albumin and urea
- Cells express a range of key hepatitis B & C markers
- Cells demonstrate LDL uptake
Hepatocyte cell morphology
When thawed and plated as a monolayer, Def-HEP cells form hepatocytes with characteristic cobblestone morphology and tight cell junctions
Hepatocyte maturation markers
QPCR analysis shows Def-HEP cells show key hepatocyte markers at similar levels to PHH. Functional characteristics including albumin secretion, A1AT production, glycogen storage and LDL uptake are also present.
Extended culture time
Multiple Inducible CYP450 Activities
Def-HEP cells display CYP450 induced activity profiles that are similar to PHH (CYP1A2 EROD assay, inducer - omeprazole), (CYP3A4 PGlo assay, inducer - rifampicin).
Hepatitis marker analysis
As assessed by dosing Def-HEP WT with valinomycin (0.3-600nM) and papverine (0.03 – 60µM) for 48 hrs both compounds caused significant mitochondrial toxicity with concentration ranges recognized in literature. Cell viability was assessed via the CellTiter 96® Aqueous Non-Radioactive Cell. Both valinomycin and papaverine caused significant mitochondrial toxicity