The Def-PANC cell products are highly functional human induced pluripotent stem (hIPS)-derived pancreatic cells. Yamanaka iPS technology in combination with fully defined differentiation conditions enables the generation of standardized populations of pancreatic cell products. Through a 25 day differentiation process the cells proceed through key developmental stages ultimately producing functional pancreatic cells.
Figure 1. When grown in standard laboratory 96-well plates Def-PANC cells show typically tight-packed pancreatic cell morphology (A) and a high proportion of C-peptide secretion (green) from beta cells (B). Panel C depicts DAPI staining of nuclear DNA (blue) as well as C-peptide (green).
When grown on 96 well low adherent plates the Def-PANC cells aggregate and form microislet structures of similar size to primary human islets.
Figure 2. Microislet formation of Def-PANC in low adherent plates.
The cells exhibit a GSIS (Glucose Stimulated Insulin Secretion) response comparable to primary islets and they have also demonstrated a dose-dependent response to well-known secretagogues such as GLP-1 and Exenatide.
Glucose Stimulated Insulin Secretion Assay
Glucose stimulated insulin secretion (GSIS) for islet-like structure thawed from a cryopreserved vial in 96 low adherent well plates.
Figure 3. Left hand panel shows GSIS assay for Def-PANC cells stimulated with low and high concentrations of glucose only/ Right hand panel overviews GSIS response of Def-PANC cells stimulated with low and high concentrations of glucose and exenatide. Low glucose concentrations 1.6mM, High glucose concentrations 16.7mM, exenatide concentration 25nM.
Detection of pancreatic INS gene expression marker by qPCR
Figure 4. Insulin gene expression marker analysis for Def-PANC cells thawed from a cryopreserved vial in 96 low adherent well plates and cultured as micro-islets.
Brochure: Definigen Pancreatic Cells Wild Type
Scientific Poster:Poster_Definigen_ISSCR 2016
We recommend that you use our online system to identify the products of interest and their respective product codes. To order we will require the following information:
Product code(s) of the item(s) you wish to order (e.g. Def-PANC WT)
Your name, email address, title and organization
Shipping address and billing address with contact names
Purchase Order number if you have one
For further information please contact us: