DefiniGEN's wild-type pancreatic beta cells are functional human pancreatic cells displaying many of the characteristics of primary human beta cells. The resulting cells display a robust Glucose Sensitive Insulin Secretion (GSIS) response over a range of physiologically relevant glucose concentrations and exhibit an elevated GSIS response to key reference drugs in a similar manner to primary human pancreatic islets. Def-PANC WT cells can be grown in monolayer, or they can be conveniently cultured as microislets which resemble primary human pancreati islets in structure and function. The cells are available for key applications including drug discovery and Diabetes research.
- Highly standardized cell product containing >97% human pancreatic cells with consistent performance and biologically relevant data
- Wild-type donor genetics and karyotype verified
- Glucose sensitive insulin secretion (GSIS) response over a range of physiologically relevant glucose concentrations
- Dose dependent GSIS response to key diabetes reference drugs
Quantification of the pancreatic insulin gene expression marker by qPCR
Def-PANC cells express the insulin gene at very similar levels to primary human pancreatic islets.
Glucose Stimulated Insulin Secretion Assay
A robust GSIS response is observed in Def-PANC cells when thawed from a cryopreserved vial in 96 well low adherent plates and grown as islet-like structures. They have also demonstrated a dose-dependent response to well-known secretagogues such as GLP-1 and Exenatide.
The Def-PANC cell products are highly functional iPSC-derived pancreataic cells. Yamanaka iPSC technology in combination with fully defined differentiation conditions enables the generation of standardized populations of pancreatic cell products. Through a 25 day differentiation process the cells proceed through key developmental stages ultimately producing functional pancreatic cells (Figure 3).
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When grown on 96-well low adherent plates the Def-PANC cells aggregate and form microislet structures of similar size to primary human islets (Figure 4).