Chief Scientific Officer Professor Ludovic Vallier previously established the Cambridge hIPSC Core Facility and Sanger hIPSC platform – generating 100’s lines for the European Stem Cell bank. Our iPS reprogramming platform is modelled on these high performance facilities. DefiniGEN are a partner in European stem cell bank deriving and validating iPS lines for both pharma and academia. We use integrative and non-integrative methods to reprogram a range of human blood or fibroblast samples generating hIPSC which are fully QCed.
5 step process for the generation of your hIPSC lines
We can then use complementary services including CRISPR gene-editing and differentiation using our OptiDIFF platform to generate custom models for clients
iPS Reprogramming projects are typically completed within 2-3 months. Actual timelines will vary depending on the unique aspects of each bespoke project.
Selected platform publications
Generation of Human Induced Pluripotent Stem Cells from Peripheral Blood Mononuclear Cells Using Sendai Virus. Soares FA, Pedersen RA, Vallier L. Methods Mol Biol. 2016;1357:23-31
Genetic background drives transcriptional variation in human induced pluripotent stem cells. Rouhani F, Kumasaka N, de Brito MC, Bradley A, Vallier L, Gaffney D. PLoS Genet. 2014 Jun 5;10(6):e1004432