Delivering phenotypically relevant disease models
Industry leader in producing reliable in-vitro liver and metabolic disease models
Bespoke CRISPR disease modeling
DefiniGEN’s induced pluripotent stem cell (iPSC) OptiDIFF platform enables the generation of high-quality wild-type and disease cell models.
We can produce mature cells which recapitulate a range of disease phenotypes and are being adopted for preclinical evaluation studies. We have developed various disease models for a wide range of metabolic indications.
DefiniGEN offer the ability to develop custom models for screening or characterizing lead compounds for indications that have previously been difficult to study.
Disease Modeling expertise
When you can't find the right iPSC line or iPSC donor material to create a cell-based model for a specific disease, DefiniGEN can help.
With our years of experience generating mature, primary-like cells from iPSCs and our proficiency at using CRISPR for targeted genome editing, we can build the cells that best reflect the biology you wish to model.
Disease modeling workflow
Our custom cell model service is an integrated, end-to-end package that includes iPSC line or donor selection, the complete CRISPR editing workflow, and differentiation into the target cell type. From basic frameshift knock-out mutations to complex knock-in genetic changes, our dedicated scientific team will work collaboratively with you to design and build the most relevant cell-based model for your project.
Our workflow consists of footprint-free CRISPR/Cas9-mediated editing, single-cell cloning of the edited population, and characterization of expanded clonal cell lines to identify positive clones. The edited iPSC cells maintain pluripotency and have normal, stable karyotypes, which are important for testing downstream applications such as directed differentiation.
We work in collaboration with you to design sgRNAs and use a footprint-free CRISPR/Cas9 system to edit the iPSCs. We optimize transfection conditions before full-scale editing, and then screen the edited populations, seed single cells, and sanger sequence the clones. You select which clones to move forward with or you can provide material derived from patient donors.
We use our world-leading OptiDIFF platform to differentiate your edited iPSCs. We've transformed the "difficult" into the "routine" and have successfully differentiated multiple iPSC lines into a range of target cell types that fully recapitulate the mature cell phenotype.
Frequently asked questions
How long is the turnaround time for a CRISPR project?
It depends on the complexity of the project but generally we deliver the edited cell line in 8 weeks. If differentiation into a specific cell type is required, additional time will be necessary and will depend on the requested scale and cell type of interest.
How long does the entire gene editing process take?
The entire gene editing process takes between 8-10 weeks depending on the complexity of the project. We will advise you of the lead time when we generate your quote.
What do you provide as a technical support package?
We work as a research partner offering collaborative services. We offer bi-weekly calls with our clients and interim emails with project reports. Our team is always available to provide technical support which can include experimental design and training on best practices for handling cells. Contact us today >