The CRISPR-Cas9 system is able to achieve highly specific and precise targeting for the genetic manipulation of cells and cell lines, transforming the field of genome engineering and presenting a wealth of new possibilities for R&D.
DefiniGEN have optimized conditions for CRISPR Cas-9 genome editing on multiple patient-derived iPSC’s, and we have built up considerable experience in using the system for genomic manipulation in a range of other cell lines.
We can offer our clients an integrated end-to-end package including sgDNA design, transfection, optimisation, and clone picking/sequencing to generate a library of gene edits for client selection. From a basic frameshift knock-out mutation to complex knock-in genetic changes, our dedicated scientific team will work collaboratively with you to design the right tools to accelerate your research programs.
|Indel for gene disruption||Gene inactivation by introducing insertion or deletion||Study gene function via gene inactivation|
|Large deletions||Deletion from few base pairs to kilobase in the desired gene|
|Multiple deletions||Editing with multiple guides in a single reaction|
|Point mutations/SNPs||Introducing point mutation or correcting disease-causing mutation||Study disease causing mutations in a clinical context and protein function in a native cell biology setting|
|Large casettes integration||Introducing large casettes|
|Tag Reporter||Integration of reporters or tags|