CRISPR Modeling Service - Definigen
Scientist in Laboratory

CRISPR Modeling

CRISPR gene-editing combined with DefiniGEN’s iPSC technology platform provides an innovative tool for producing disease-relevant models

DefiniGEN Scientist in laboratory

Overview

The CRISPR-Cas9 system is able to achieve highly specific and precise targeting for the genetic manipulation of cells and cell lines, transforming the field of genome engineering and presenting a wealth of new possibilities for R&D.

Definigen test tube in lab

Why choose DefiniGEN?

DefiniGEN have optimized conditions for CRISPR Cas-9 genome editing on multiple patient-derived iPSC’s, and we have built up considerable experience in using the system for genomic manipulation in a range of other cell lines.

We can offer our clients an integrated end-to-end package including sgDNA design, transfection, optimisation, and clone picking/sequencing to generate a library of gene edits for client selection. From a basic frameshift knock-out mutation to complex knock-in genetic changes, our dedicated scientific team will work collaboratively with you to design the right tools to accelerate your research programs.

Edits available

Service Description Application
Knockout
Indel for gene disruption Gene inactivation by introducing insertion or deletion Study gene function via gene inactivation
Large deletions Deletion from few base pairs to kilobase in the desired gene
Multiple deletions Editing with multiple guides in a single reaction
Knock-in
Point mutations/SNPs Introducing point mutation or correcting disease-causing mutation Study disease causing mutations in a clinical context and protein function in a native cell biology setting
Large casettes integration Introducing large casettes
Tag Reporter Integration of reporters or tags

Workflow

We have developed a complete workflow for the knockout of an endogenous gene. Our workflow consists of footprint-free CRISPR/Cas9-mediated editing, single-cell cloning of the edited population, and characterization of expanded clonal cell lines to identify positive clones. The edited iPSC cells maintain pluripotency and have normal, stable karyotypes, which are important for testing downstream applications such as directed differentiation.