Our expertise can help design the guide RNA’s (gRNA) in silico for maximum efficiency, then optimize the electroporation of the cell line in question to best establish ideal conditions for the gene edit. Our team then methodically sequence a number of clones to confirm the precise genetic manipulation has been successful.
|Indel for gene disruption||Gene inactivation by introducing insertion or deletion||Study gene function via gene inactivation|
|Large deletions||Deletion from few base pairs to kilobase in the desired gene|
|Multiple deletions||Editing with multiple guides in a single reaction|
|Point mutations/SNPs||Introducing point mutation or correcting disease-causing mutation||Study disease causing mutations in a clinical context and protein function in a native cell biology setting|
|Large cassettes integration||Introducing large cassettes|
|Tag Reporter||Integration of reporters or tags|