IPSC Differentiation
Advance drug discovery with physiologically relevant human disease models
Our iPSC Differentiation Service
With the power to deliver virtually unlimited amounts of genetically-identical, primary-like human cells, iPSCs are an exciting technology. They open the door to more physiologically relevant models of human disease than animal cell-based models, and enable highly reproducible results by removing donor-to-donor variability. But differentiating iPSCs into target cells can be challenging, which is why many labs turn to DefiniGEN.
At DefiniGEN, we have transformed the "difficult" into the "routine" and have successfully differentiated multiple iPSC lines into a range of target cell types that fully recapitulate the mature cell phenotype. Whatever cell type your project needs, even if it's one of the more difficult targets like hepatocytes or pancreatic cells, DefiniGEN has the expertise to quickly deliver.
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Significantly Higherdifferentiation success rates compared to standard protocols |
8 - 10 Weekstypical turnaround time for our differentiation projects |
7+ Yearsof successfully completed projects |
What you can expect
We provide ongoing communication and full transparency during every stage of your project.
*Using our in-house iPSCs can increase your chances of success as we already have a deep understanding of how these iPSCs will behave.
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1) iPSC Reprogramming or SourcingWe can start your service with any of our comprehensively characterized in-house iPSC lines*, you can supply your own iPSC lines, or you can provide material derived from patient donors. We can even help you find the right donor material from our extensive clinical network. -
2) Differentiation and bankingWe use our world-leading optiDIFF platform to differentiate your iPSCs. Once the differentiation process is complete, we cryopreserve and bank the differentiated cells. -
3) Product ValidationWe use a variety of analytical tools, including genotyping, phenotyping, and cell-based assays, to ensure that the target cells possess the expected characteristics of the mature cell. -
4) DeliveryUpon successful validation, we ship your cryopreserved differentiated cells to you, and you can expect high viability post-thaw.
Why choose DefiniGEN?
Our iPSC differentiation services deliver high-quality bespoke cell models, especially in support of metabolic and rare disease projects. Our workflow includes everything from sourcing to differentiation to validation and more, and each service is fully customized to meet your specific project's needs.
Contact us to learn more about working with DefiniGEN - we respond within 1 business day to every enquiry >
We are an approved supplier on scientist.com
Small Scale Optimization
The source of our high success rates lies in an initial small-scale optimization step. By optimizing the differentiation process on a small scale, we can account for the inherent variability of different iPSC lines and maximize the efficiency of our proprietary, highly scalable OptiDIFF platform.
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Small-scale optimizationBecause each iPSC line responds differently to the differentiation process, we start each project with a small-scale optimization.
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Condition findingWe evaluate multiple parameters in parallel to quickly identify the best differentiation conditions for the specific iPSC line and target cell type.
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Full-scale productionWe quickly identify the conditions needed to increase the chances of successful full-scale production. Typical project turnaround time are as short as 8 weeks.
Our Services are tailored to scientists who...
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Want rapid turnaround and high quality
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Want to work with human cell models for increased fidelity
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Don't want to rely on primary cells because of time constraints
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Work with difficult-to-model diseases
Frequently Asked Questions
iPSC Differentiation
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We work as a research partner offering collaborative services. We offer bi-weekly calls with our clients and interim emails with project reports. Our team is always available to provide technical support which can include experimental design and training on best practices for handling cells. Contact us today >
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To generate a quote, we need your name, the name of your organization, your email address and details on the gene, mutation of interest, and cell type to edit. We also recommend contacting our team to go through project requirements in more detail. Contact us today >