iPSC Derived Hepatocytes

DefiniGEN’s induced pluripotent stem cell (iPSC) differentiation platform generates high quality wild-type and disease cell models. We can produce mature hepatocytes which recapitulate a range of disease phenotypes. We provide off-the-shelf iPSC derived hepatocytes, but we also offer the ability to model various diseases.

Our hepatocytes are highly functional human hepatocyte cells which have normal genetics (wild type donor + karotype verified). The cells display the functional characteristics of primary human hepatocytes including: albumin production, A1AT secretion, glycogen storage, CYP450 expression.

 

 

Hepatocyte WT        Hepatocyte NAFLD    Hepatocyte A1ATD      Hepatocyte GSD1a       Hepatocyte FH

 

Generation of Hepatocytes from Pluripotent Stem Cells for Drug Screening and Developmental Modeling. Gieseck RL 3rd, Vallier L, Hannan NR. Methods Mol Biol. 2015;1250:123-42.

Maturation of induced pluripotent stem cell derived hepatocytes by 3D-culture. GieseckRL 3rd, Hannan NR, Vallier L. PLoS One. 2014 Jan 22;9(1):e86372.

Production of hepatocyte-like cells from human pluripotent stem cells. Hannan NR, Vallier L et al. Nature Protocols.2013 Feb;8(2):430-7.

Targeted gene correction of α1-antitrypsin deficiency in induced pluripotent stem cells.Yusa K, Rashid ST, Vallier L, et al. Nature. 2011 Oct 12;478(7369):391-4.

Modeling inherited metabolic disorders of the liver using human induced pluripotent stem cells. Rashid ST, Lomas DA, Vallier L et al. J Clin Invest. 2010 Sep;120(9):3127-36.